Fig. 4: Adeno-associated virus-mediated overexpression of PDE4D exacerbated renal injury. | Cell Death & Disease

Fig. 4: Adeno-associated virus-mediated overexpression of PDE4D exacerbated renal injury.

From: Genetic deletion of phosphodiesterase 4D in the liver improves kidney damage in high-fat fed mice: liver-kidney crosstalk

Fig. 4

AAV8-PDE4D- and AAV8-GFP-treated mice were fed normal chow for 4 weeks. A The relative mRNA levels of PDE4D in the livers of the two groups. B, C ITT (B) and GTT (C) were performed on the AAV8-GFP and AAV8-PDE4D groups at week 4, and the AUCs of GTT and ITT were calculated. D Phosphorylation of AKT in liver (left), epididymal fat (center), and gastrocnemius muscle (right) after mice were injected with insulin for 15 min (n = 5–6). E The protein levels of PDE4D and p-AKT/AKT in kidney tissue of AAV8-GFP and AAV8-PDE4D mice (n = 5–6). F Kidney weight of the two groups of mice. G Serum creatinine and BUN levels of the two groups of mice. H HE, Oil red O, PAS, and Sirius red staining of mouse kidney. I TUNEL staining in kidney tissue of AAV8-GFP and AAV8-PDE4D mice. Data are means ± SEMs. *P < 0.05, **P < 0.01, ***P < 0.001 with a two-tailed Student’s t test.

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