Fig. 5: Hepatic PDE4D mediated NAFLD-associated renal injury through the TGF-β1 pathway. | Cell Death & Disease

Fig. 5: Hepatic PDE4D mediated NAFLD-associated renal injury through the TGF-β1 pathway.

From: Genetic deletion of phosphodiesterase 4D in the liver improves kidney damage in high-fat fed mice: liver-kidney crosstalk

Fig. 5

A The protein levels of TGF-β1 and CD36 in the livers of WT and PDE4D-LKO mice. B The protein levels of TGF-β1, p-SMAD2, and SMAD2 in the kidneys of WT and PDE4D-LKO HFD mice. C The concentration of TGF-β1 in the serum of WT and PDE4D-LKO mice fed with HFD for 16 weeks was detected by ELISA. D The protein levels of TGF-β1, p-SMAD2, and SMAD2 in the kidneys of AAV8-GFP and AAV8-PDE4D mice. E, F HK-2 cells were treated with the supernatant of hepatocytes infected with PDE4D adenovirus (PDE4D-Ad) or empty adenovirus (EV-Ad). SB431542 was used to inhibit TGF-β1 signaling. The mRNA levels of TGF-β1 in HK-2 cells were detected by RT-qPCR. The expression of TGF-β1 and collagen1 were detected by western blot. Data are shown as the means ± SEMs. *P < 0.05, **P < 0.01, ***P < 0.001 with a two-tailed Student’s t test.

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