Fig. 6: The PDE4 inhibitor roflumilast alleviated renal injury induced by a high-fat diet. | Cell Death & Disease

Fig. 6: The PDE4 inhibitor roflumilast alleviated renal injury induced by a high-fat diet.

From: Genetic deletion of phosphodiesterase 4D in the liver improves kidney damage in high-fat fed mice: liver-kidney crosstalk

Fig. 6

Roflumilast treatment (Rof-Therapy) started from 16 weeks post-HFD and lasted for 4 weeks. A Kidney weight of HFD and Rof-Therapy mice. B Serum creatinine (left) and BUN (right) levels of the two groups of mice. C HE, Oil red O, PAS, and Sirius red staining of mouse kidney. D TUNEL staining in kidney tissue of HFD and Rof-Therapy mice. E Western blot analysis of the protein levels of PDE4D in the kidneys of the two groups of mice. F Western blot analysis of the protein levels of TGF-β1 and p-SMAD2/SMAD2 in the kidneys of the two groups of mice. Data are shown as the means ± SEMs. *P < 0.05, **P < 0.01, ***P < 0.001 with a two-tailed Student’s t test.

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