Table 1 Primer list.

From: CHST2-mediated sulfation of MECA79 antigens is critical for breast cancer cell migration and metastasis

 

Primers

Sequences

Sense sequences in shRNA

CHST2 shRNA-1

ATACAACTGGAAGACAGAGAG

 

CHST2 shRNA-2

TTTGATCTGCTGGAAGGTGAG

Primers for ChIP

CHST2-1 Forward

GCCACAGACCCACTAGAGAG

 

CHST2-1 Reverse

GAGAGTTGGATTCTGCGTGC

 

CHST2-2 Forward

CACCTGGGTTTCCTTGCCTA

 

CHST2-2 Reverse

GAGAGGTGGGAAAATTCGCC

 

CHST2-5k Forward

CAATCAAGCCCAAGGAAAGA

 

CHST2-5k Reverse

ACAGGCTGGCTTACTGCCTA

Primers for qRT-PCR

Snail-84-Forward

TTTACCTTCCAGCAGCCCTA

 

Snail-291-Reverse

CCCACTGTCCTCATCTGACA

 

CHST2-1322- Forward

TTTTGTGGGACTGTTGGTGA

 

CHST2-1485- Reverse

CACCTGTTTGATCTGCTGGA

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