Fig. 7: KRT7-AS elevates amounts of the key tumor suppressor PTEN in cancer cells and inhibits tumorigenesis. | Cell Death & Disease

Fig. 7: KRT7-AS elevates amounts of the key tumor suppressor PTEN in cancer cells and inhibits tumorigenesis.

From: The long non-coding RNA keratin-7 antisense acts as a new tumor suppressor to inhibit tumorigenesis and enhance apoptosis in lung and breast cancers

Fig. 7

Western blotting showed that overexpression of KRT7-AS elevated PTEN protein levels in SPC-A-1 and H1299 lung cancer cells (A), and in MCF-7 breast cancer cells (B), whereas silencing of KRT7-AS reduced the levels of PTEN in A549 lung cancer cells (B). The effect of either overexpression of KRT7-AS or silencing of KRT7-AS on PTEN levels in SPC-A-1 cells (C) and A549 lung cancer cells (D) was accessed by IF staining. Expression of PTEN in lung cancer (E, G) and breast cancer tissues (F, G), and their paired normal tissues (E–G) was detected by Western blotting. PTEN protein levels in lung cancer (H, J) and breast cancer (I, J) tissues were measured by IHC staining. The effect of KRT7-AS on PTEN levels in the tumor tissues from the xenograft mice was accessed by western blotting (K–N) and IHC staining (O–R), respectively. Cell viability and colony formation were detected in KRT7-AS-silenced or PTEN re-overexpressed A549 cells, and control cells (S–U). Data are shown as mean ± SD of three independent replicates. **P < 0.01, ***P < 0.001.

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