Fig. 7: Establishment of the microglial C3aR antagonist delivery system.
A Representative TEM images of MCNPs@SB. Scale bars: 100 nm. B, C Particle size and Zeta potential analysis of NPs, NPs@SB, CNPs@SB, and MCNPs@SB; n = 3 per group. D Z-average size, PDI, EE% and LE% of NPs, NPs@SB, CNPs@SB and MCNPs@SB; n = 3 per group. E The drug release kinetics of SB290157 and MCNPs@SB; n = 3 per group. F, G Representative images and quantitative fluorescence analysis of lysosomal tracker (Lyso-tracker) in microglia after 3 h incubation with Cy5.5-labeled NPs@SB, CNPs@SB, and MCNPs@SB; n = 7–9. Scale bars: 20 μm. Magnified images are shown in the right columns of panel. Scale bars: 6.5 μm. H Real-time fluorescence imaging of mice after intravenous injection of Cy5.5-labeled NPs@SB, CNPs@SB, and MCNPs@SB. I The fluorescence density in the brain at different time points; n = 3 per group. J, K Ex vivo imaging and corresponding fluorescence analysis of sacrificed tissues (heart, liver, spleen, lung, kidney, and brain) at 24 h after intravenous injection of Cy5.5-labeled NPs@SB, CNPs@SB, and MCNPs@SB. L The fluorescence density in the brain at 24 h after intravenous injection of Cy5.5-labeled NPs@SB, CNPs@SB and MCNPs@SB; n = 3 per group. M Representative images of Iba1 and GFAP staining at 6 h post-injection in SNpc derived from mice treated with Cy5.5-labeled NPs@SB, CNPs@SB and MCNPs@SB. Scale bars: 20 μm. Magnified images are shown in the right column of the panels. Scale bars: 6.5 μm. White arrows in the enlarged details show the presence of nanoparticles in microglia. N Analysis of the fluorescence density of microglial Cy5.5; n = 7–8. Results are expressed as the mean ± SEM. **p < 0.01, *p < 0.05 vs. NPs@SB; ##p < 0.01 vs. CNPs@SB. Statistical significance was determined using one-way ANOVA + Tukey’s multiple comparisons test.
