Fig. 1: KLF12 has an important role in breast cancer’s proliferation, cell cycle, and apoptosis. | Cell Death & Disease

Fig. 1: KLF12 has an important role in breast cancer’s proliferation, cell cycle, and apoptosis.

From: KLF12 promotes the proliferation of breast cancer cells by reducing the transcription of p21 in a p53-dependent and p53-independent manner

Fig. 1

a The expression of KLF12 in Hela, MCF10A, ZR-75-30, MCF-7, MBA-MD-231, and T47D cells by western blot assay. b MTT assay showing the growth curves of MCF-7 cells transfected with GFP-KLF12 or the control vector. Data are presented as means ± SDs. (p < 0.05, significant; ns, not significant; *p < 0.05; **p < 0.01). c MTT assay showing the growth curves of ZR-75-30 cells transfected with NC, shKLF12#1, and shKLF12#2. Data are presented as means ± SDs. (p < 0.05, significant; ns, not significant; *p < 0.05; **p < 0.01). d The effect of KLF12 on the cell cycle of breast cancer cell lines by flow cytometry assay. MCF-7 cells were transfected with GFP-KLF12 or the control vector. ZR-75-30 cells were transfected with NC, shKLF12#1, and shKLF12#2. The histogram on the right shows the percentage of different phase cells. e The effect of KLF12 on cellular apoptosis by flow cytometry assay. MCF-7 cells were transfected with GFP-KLF12 or the control vector. ZR-75-30 cells were transfected with NC, shKLF12#1, and shKLF12#2. The histogram on the right shows the percentage of early apoptotic cells.

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