Fig. 5: KLF12 reduces p53 protein levels by inhibiting the acetylation of p53 by p300. | Cell Death & Disease

Fig. 5: KLF12 reduces p53 protein levels by inhibiting the acetylation of p53 by p300.

From: KLF12 promotes the proliferation of breast cancer cells by reducing the transcription of p21 in a p53-dependent and p53-independent manner

Fig. 5

a CoIP assay showed the effect of KLF12 on the acetylation of endogenous p53 in MCF-7 cells. The endogenous p53 was coimmunoprecipitated with anti-p53 and then subjected to western blot analysis. b The effect of KLF12 on the protein levels of wild-type p53, p53K370/372/373R, and p53K381/382/386R in HEK293T cells by Western blot assay. c The effect of KLF12 on the acetylation of p53 and p53K370/372/373R in HEK293T cells by CoIP assay. Flag-p53 and Flag-p53K370/372/373R were coimmunoprecipitated with anti-Flag antibodies and then subjected to western blot analysis. d CoIP assay showed the changes in ubiquitin levels linked to Flag-p53 and Flag-p53K370/372/373R in HEK293T cells. e CoIP assay showed an interaction between exogenous KLF12 and p300 in HEK293T cells. GFP-KLF12 was coimmunoprecipitated with anti-GFP and then subjected to western blot analysis. f CoIP assay detected the effect of KLF12 on the interaction between p300 with p53 in HEK293T cells. HEK293T cells were transfected with indicated plasmids. g GST-pulldown assay showed KLF12 inhibited the interaction of purified GST-p53 with p300 in vitro. HEK293T cells were transfected with indicated plasmids.

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