Fig. 3: Apoptosis induced by Chiauranib in NKTL cell lines was not promoted by the classical mitochondrial apoptosis pathway.

A Protein extracts from SNK6 cells were analyzed by immunoblotting for cleaved caspase 8, 9, 3, and β-actin. SNK6 cells were treated with DMSO or Chiauranib for 24 h. B Immunoblotting analysis of mitochondria and cytosol enriched fractions of SNK6 cells treated with Chiauranib or DMSO for 24 h. C, D Annexin V-PI staining of SNK6 cells treated by DMSO or Chiauranib with or without Z-VAD-FMK (50 μM).