Fig. 5: Chiauranib promotes AIF release into the nucleus via the AKT-GSK3B-VDAC pathway, thereby inducing apoptosis. | Cell Death & Disease

Fig. 5: Chiauranib promotes AIF release into the nucleus via the AKT-GSK3B-VDAC pathway, thereby inducing apoptosis.

From: Inhibition of extranodal NK/T-cell lymphoma by Chiauranib through an AIF-dependent pathway and its synergy with L-asparaginase

Fig. 5

A–D SNK6 cells were treated with Chiauranib or DMSO for 24 h. SNK6 were pretreated with 5 µM SC-79 or 5 mM LiCl for 30 min before Chiauranib treatment. Immunoblotting analysis of the AKT-GSK3B-VDAC pathway was performed. C, D Immunoblotting analysis of mitochondria and nucleus enriched fractions of SNK6 cells. siNC (control) and siVDAC-3 was transfected into SNK6 before experiment. The efficacy of siRNA is shown in Supplementary Fig. 1B. E, F Annexin V-PI staining of SNK6 cells treated with Chiauranib or DMSO for 24 h with or without SC-79, LiCl.

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