Fig. 1: TIM-3 expressed by DCs promotes cDC2 abundance in chronically infected mice. | Cell Death & Disease

Fig. 1: TIM-3 expressed by DCs promotes cDC2 abundance in chronically infected mice.

From: TIM-3 increases the abundance of type-2 dendritic cells during Leishmania donovani infection by enhancing IL-10 production via STAT3

Fig. 1

A The frequency (left) and number [per 1.5 × 104 DCs (i.e., CD11c+ gated cells); right] of cDC1 (CD11c+CD11b−CD8α+) and cDC2 (CD11c+CD11b+CD8α−) in splenocytes prepared from uninfected (UI) and LD-infected (Inf) mice; assessed on day 45 postinfection by flow cytometry (representative data of n = 6; left). Numbers in quadrants indicate the percent cells in each throughout. Right, bar graphs show compiled data (n = 6 mice per group). B Expression of IRF4, KLF4, IRF8, and BATF3 mRNA in sDCs (i.e., CD11c+ spleen cells) that were magnetically sorted from splenocytes of uninfected and 45-day-infected mice; analyzed by quantitative RT-PCR. Results were normalized to the expression of ACTB mRNA (encoding β-actin) and are presented as fold change relative to sDCs of uninfected mice (n = 6 replicates per group). C A representative immunoblot (out of n = 2) showing the expression of TIM-3 and β-actin (loading control) in BMDCs left untransfected (−siRNA) or transfected with control (Ctrl) siRNA or TIM-3-specific siRNA. D Experimental schematic for adoptive transfer of TIM-3-silenced BMDCs: BALB/c BMDCs (1 × 106) were transfected with siRNAs as in panel (C) and then transferred intravenously into LD-infected BALB/c mice on indicated days postinfection. Alternatively, both uninfected and LD-infected mice were left without any DC transfer. On day 48 postinfection, the spleens of these mice were isolated for analyses indicated in panel (E). E The frequency (left) and number (per 1.5 × 104 CD11c+ DCs; right) of cDC1 and cDC2 in splenocytes of uninfected and LD-infected mice treated as in panel (D); measured via flow cytometry on day 48 postinfection. Left panel, representative data of n = 6. Right panel, compiled data (n = 6 mice per group). See Supplementary Fig. 2A, which shows the efficiency of TIM-3 silencing in BMDCs localized in the spleens of recipient mice after one day of transfer. Each symbol in (A) (right panel), (B), and (E) (right panel) represents data of an individual mouse; bars (in panel (B)) indicate means. Error bars indicate SD. **p < 0.01, ***p < 0.001; NS, not significant.

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