Fig. 1: Longitudinal blood, histopathological and biochemical analysis of liver fibrosis dynamics.
From: Tolerance of repeated toxic injuries of murine livers is associated with steatosis and inflammation
a Experimental setup using carbon tetrachloride (CCl4) administration in mice twice per week for 10 weeks. Blood and liver were collected weekly for further multi-level analysis. Oil-treated mice of weeks zero and ten were used as control. b Kaplan–Meier curve for survival analysis. c Longitudinal blood-based analysis of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycerides, and cholesterol. Results are presented as the mean of 3–6 mice ± SD per week, and a significant difference to control is denoted via *p < 0.05, **p < 0.01. d Cytochrome P4502e1 (CYP2E1), alpha-smooth muscle actin (α-SMA), picro-sirius red (PSR), and hematoxylin & eosin (HE) staining with positive quantified signals as a percentage of total area. Scale bars are 100 µm. e Biochemical analysis of the Hydroxyproline level development over the ten-week treatment period.
