Fig. 2: KLF6 knockdown exacerbates hepatocellular injury and inflammation during hepatic I/R.
From: KLF6 alleviates hepatic ischemia-reperfusion injury by inhibiting autophagy
a, b Male C57BL/6N mice at 6–8 weeks of age were infected with KLF6 shRNA adenovirus via tail-vein injection. Seven days after the adenovirus injection, KLF6 expression was assessed by RT-qPCR and western blotting analysis. c Representative histological H&E-stained images showing necrotic areas in liver tissue from AD-shNC and AD-shKLF6 mice after hepatic I/R. Scale bar, 500 μm. d Serum ALT/AST/LDH levels of AD-shNC and AD-shKLF6 mice after hepatic I/R. e, f Representative pictures of liver stained with Ly-6G (neutrophils) and F4/80 (macrophages). Scale bar, 50 μm. g TUNEL staining in liver sections from AD-shNC and AD-shKLF6 mice after I/R. Scale bar, 20 μm. Each group includes six mice. Mean values ± SD are shown, and statistical significance was determined by two-tailed Student’s t test. *p < 0.05.
