Fig. 4: KLF6 deficiency aggravates H/R‑induced AML12 cell injury.
From: KLF6 alleviates hepatic ischemia-reperfusion injury by inhibiting autophagy
a Left panel, schematic of CRISPR/Cas9-mediated knockout of Klf6 in AML12 cells. Right panel, western blotting analysis of KLF6 expression in WT and KO AML12 cells. b WT and KO cells were exposed to 1% O2 for 6 h, and then they were transferred to normoxia and the cell viability was measured by CCK8 kit at different time points after reoxygenation. c Expression of cleaved caspase3, BAX and Bcl2 were detected by western blot analysis. d Left panel, representative flow cytometry plots of annexin V/PI stained cells. Right panel, statistical analysis of total apoptotic cells in different groups. e Nuclei were visualized with Hoechst 33342. Apoptotic cells were distinguished by the presence of bright blue nuclei. Scale bar, 200 μm. f In situ fluorescent staining (red) detection of ROS in KLF6 WT and KO cells after H/R (Left panel) and quantified average fluorescence intensity by ImageJ (Right panel). Scale bar, 100 μm. Mean values ± SD are shown, and statistical significance was determined by one-way ANOVA followed by Dunnett’s multiple comparison test. *p < 0.05.
