Fig. 7: The regulatory effects of KLF6 on H/R injury in AML12 cells are autophagy dependent.
From: KLF6 alleviates hepatic ischemia-reperfusion injury by inhibiting autophagy
a The autophagic flux of mCherry-GFP-LC3 transfected WT and KO cells after normoxic control or H/R was revealed using laser confocal microscopy (left panel) and assessed by visual screening (right panel). Scale bar, 10 μm. b, c Cells were pre-treated with 3-MA (5 mmol/l, 1 h) or RAPA (10 μmol/l, 24 h) and then subjected to H/R. Cell viability and LDH release were evaluated with the CCK8 Assay Kit and LDH Assay Kit, respectively. Mean values ± SD are shown, and statistical significance was determined by two-tailed Student’s t test. *p < 0.05.
