Fig. 2: Confirmation of the involvement of UFL1 and UFSP2 in RPL10 ufmylation and the effect on pancreatic cancer cells.
From: The ufmylation modification of ribosomal protein L10 in the development of pancreatic adenocarcinoma
A Direct binding of RPL10 and UFL1 in PANC-1 cells was detected by Co-IP. B The change of RPL10 ufmylation was detected by after UFL1 silencing in PANC-1 cells. C Direct binding of UFSP2 and RPL10 in PANC-1 cells by Co-IP. D The change of RPL10 ufmylation after overexpressing UFSP2 in PANC-1 cells. E The proliferations of PANC-1 cells and Mia PaCa-2 cells after silencing UFL1. The proliferations were detected by CCK-8 method (n = 10). F The proliferations of PANC-1 and Mia PaCa-2 cells after overexpressing UFSP2. The proliferation rates were detected by CCK-8 method (n = 10). G The colony formation of PANC-1 and Mia PaCa-2 cells after silencing UFL1. The colonies were stained with crystal violet and analyzed (n = 3). H The colony formation of PANC-1 and Mia PaCa-2 cells after overexpressing UFSP2. The colonies were stained with crystal violet and analyzed (n = 3). All experiments were independently repeated for 3 times, and the data are expressed as mean ± SD. *P < 0.05 indicates statistically significant versus negative control. **P < 0.01 indicates statistically very significant versus negative control.
