Fig. 1: LINC00641 expression is down‐regulated in LUAD and its characterization. | Cell Death & Disease

Fig. 1: LINC00641 expression is down‐regulated in LUAD and its characterization.

From: Downregulation of N6-methyladenosine-modified LINC00641 promotes EMT, but provides a ferroptotic vulnerability in lung cancer

Fig. 1

A LINC00641 expression data in TCGA LUAD dataset (58 normal vs 488 tumor tissues) was downloaded from the TANRIC website (https://ibl.mdanderson.org/tanric/_design/basic/main.html). The results were expressed as the mean ± SEM. **Mann–Whitney U‐test, P < 0.01. B LINC00641 expression in tumor tissues of different TNM stages in TCGA LUAD dataset. The results were expressed as the mean ± SEM. **Kruskal Wallis test, P < 0.01. C Kaplan–Meier curves for relapse free survival (RFS) of LUAD patients expressing high and low expression levels of LINC00641 in TCGA LUAD dataset. *Log‐rank test, P < 0.05. D Rapid amplification of cDNA ends (RACE) assays in A549 cells to detect the whole sequence of LINC00641. Left: sequencing of PCR products indicated the boundary between the universal anchor primer and LINC00641 sequences. Right: a gel electrophoresis image of PCR products from the 5′-RACE and 3′-RACE assays. E The coding potential of LINC00641. Coding potentials of lncRNA (LINC00641, MALAT1 and lncRNA-LET) and mRNA (GAPDH, ACTB) were calculated using CPAT (https://wlcb.oit.uci.edu/cpat/) and CPC2 (http://cpc2.gao-lab.org/index.php). F The subcellular localization of LINC00641 in LncATLAS database. G qRT-PCR detection of the percentage of LINC00641 and GAPDH in the nuclear and cytoplasmic fractions of A549 and H1299 cells. GAPDH served as the cytoplasmic localization control.

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