Fig. 3: TOPK interacts with YB1 in EC cells. | Cell Death & Disease

Fig. 3: TOPK interacts with YB1 in EC cells.

From: TOPK promotes the growth of esophageal cancer in vitro and in vivo by enhancing YB1/eEF1A1 signal pathway

Fig. 3

a, b The correlation between TOPK and YB1 protein in EC were detected by Western blot (a) and analyzed by image J (b). c Bimolecular fluorescence complementation (BiFC) assay was used to detect the binding between TOPK and YB1 in vitro. d Immunofluorescence was used to detect the binding between TOPK and YB1 in KYSE150 and KYSE30. e, f KYSE150 (e) and KYSE30 (f) cells were immunoprecipitated with anti-TOPK antibodies. After immunoprecipitation (IP) with anti-TOPK or IgG antibodies, proteins were separated by SDS-PAGE and detected by immunoblotting (IB) with anti-YB1 antibodies. The lysate was also shown as input. g, h KYSE150 (g) and KYSE30 (h) cells were immunoprecipitated with anti-YB1 antibodies. After IP with anti-YB1 or IgG antibodies, proteins were separated by SDS-PAGE and detected by immunoblotting with anti-TOPK antibodies. The lysate was also shown as input. i, j KYSE150 (i) and KYSE30 (j) cells with stable knockout of TOPK were immunoprecipitated with anti-TOPK. After IP with anti-TOPK or IgG antibodies, proteins were separated by SDS-PAGE and detected by immunoblotting with anti-YB1 antibodies. The lysate was also shown as input. All experiments were biological replicates and were repeated at least three times. Error bars showed standard error of the mean. *p ˂ 0.05, **p ˂ 0.01.

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