Fig. 4: TOPK promotes cell proliferation by phosphorylating YB1 at T89 and S209. | Cell Death & Disease

Fig. 4: TOPK promotes cell proliferation by phosphorylating YB1 at T89 and S209.

From: TOPK promotes the growth of esophageal cancer in vitro and in vivo by enhancing YB1/eEF1A1 signal pathway

Fig. 4

a YB1 kinase activity was assessed by an in vitro kinase assay using active TOPK and inactive YB1 proteins. The effect of TOPK was determined by Western blot. b, c The proliferation ability of KYSE150 (b) and KYSE30 (c) were recovered after YB1 was rescued in sgTOPK KYSE150 and KYSE30 cells. d, e The anchorage-independent growth ability of KYSE150 (d) and KYSE30 (e) were recovered after YB1 was rescued in sgTOPK KYSE150 and KYSE30 cells. f, g The anchorage-dependent growth ability of KYSE150 (f) and KYSE30 (g) were recovered after YB1 was rescued in sgTOPK KYSE150 and KYSE30 cells. h The phosphorylation ability of TOPK on YB1 was analyzed by in vitro kinase assay after mutation of T89 and S209 sites in YB1. i, j The protein level of YB1 T89A and S209A were detected in KYSE150 sgTOPK (i) and KYSE30 sgTOPK (j) cells by Western blot. All experiments were biological replicates and were repeated at least three times. Error bars showed standard error of the mean. *p ˂ 0.05, **p ˂ 0.01, ***p ˂ 0.001.

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