Fig. 5: TOPK inhibits YB1 binding to the promoter of eEF1A1. | Cell Death & Disease

Fig. 5: TOPK inhibits YB1 binding to the promoter of eEF1A1.

From: TOPK promotes the growth of esophageal cancer in vitro and in vivo by enhancing YB1/eEF1A1 signal pathway

Fig. 5

a EEF1A1 was the top putative target of YB1 by CHIP-seq in HEK293 cell in GEO data (GSM1151769). b The schematic diagram of different binding sequences in the promoter of eEF1A1 designed for the dual-luciferase reporter assay. c The binding sequence between YB1 and eEF1A1 promoter was identified by luciferase activity assay. d The binding ability between YB1 and eEF1A1 promoter sequence was inhibited by TOPK inhibitor HI-TOPK-032. e The binding ability between YB1 and eEF1A1 promoter sequence was suppressed after YB1 mutation. f, g The mRNA level of eEF1A1 was identified in TOPK knockout KYSE150 (f) and KYSE30 (g) cells by q-PCR. h, i The general translational level of protein synthesis was confirmed in the sgTOPK KYSE150 (h) and KYSE30 (i) cells by protein synthesis assay. j, k The protein level of eEF1A1 and the phosphorylation levels of mTOR, AKT and p70S6K were identified in the sgTOPK KYSE150 (j) and KYSE30 (k) cells by Western blot. All experiments were biological replicates and were repeated at least three times. Error bars showed standard error of the mean. *p ˂ 0.05, **p ˂ 0.01, ***p ˂ 0.001.

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