Fig. 3: Oocyte maturation is impaired in oocytes from Xrcc5-cKO mice following etoposide-induced DNA damage. | Cell Death & Disease

Fig. 3: Oocyte maturation is impaired in oocytes from Xrcc5-cKO mice following etoposide-induced DNA damage.

From: Xrcc5/Ku80 is required for the repair of DNA damage in fully grown meiotically arrested mammalian oocytes

Fig. 3

A Fully grown GV oocytes were exposed to 0, 50, or 100 µg/ml etoposide for 3 h, then released from meiotic arrest and in vitro maturation (IVM) monitored. B Percentage of oocytes that underwent GVBD transition after etoposide treatment over a 6-h time period. (i) Data were generated from three independent experiments; (ii)–(iv) are drawn from (i); number of oocytes analyzed is shown in parentheses. Student’s t-test was used for statistical analyses. Error bars are mean ± SEM, *p < 0.05, **p < 0.01. C Representative immunofluorescence images showing the chromosome alignment of WT and Xrcc5-cKO oocytes 16 h after treatment with 50 µg/ml etoposide.

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