Table 1 Sequences of primers used for qRT-PCR.
Gene | Primer | Sequence (5’ → 3’) |
|---|---|---|
Mus-GAPDH | Forward | CCTCGTCCCGTAGACAAAATG |
Reverse | TGAGGTCAATGAAGGGGTCGT | |
Mus-β-actin | Forward | GGCTGTATTCCCCTCCATCG |
Reverse | CCAGTTGGTAACAATGCCATGT | |
Mus-Tspyl2 | Forward | TGACCCAGAGGAAAAGAATACC |
Reverse | CACTGCTGTCCTGGATATTAGT | |
Mus-α-SMA | Forward | GTCCCAGACATCAGGGAGTAA |
Reverse | TCGGATACTTCAGCGTCAGGA | |
Mus-Collagen I | Forward | AAGAAGCACGTCTGGTTTGGAG |
Reverse | GGTCCATGTAGGCTACGCTGTT | |
Mus-TGF-β | Forward | CAACAATTCCTGGCGTTACCTT |
Reverse | TCGAAAGCCCTGTATTCCGTCT | |
Mus-Fibronectin | Forward | GCTCAGCAAATCGTGCAGC |
Reverse | CTAGGTAGGTCCGTTCCCACT | |
Homo-GAPDH | Forward | GGTGGTCTCCTCTGACTTCAACA |
Reverse | TCTCTTCCTCTTGTGCTCTTGCT | |
Homo-Tspyl2 | Forward | AACAACAACGAGAACACTTACG |
Reverse | TATCTTCATCATCACTGGCCTC | |
Homo-α-SMA | Forward | TCGTGCTGGACTCTGGAGATGG |
Reverse | CCACGCTCAGTCAGGATCTTCATG | |
Homo-Collagen I α1 | Forward | AAAGATGGACTCAACGGTCTC |
Reverse | CATCGTGAGCCTTCTCTTGAG | |
Homo-Collagen I α2 | Forward | CTCCATGGTGAGTTTGGTCTC |
Reverse | CTTCCAATAGGACCAGTAGGAC | |
Homo-Fibronectin | Forward | GGCGACAGGACGGACATCTTTG |
Reverse | GGCACAAGGCACCATTGGAATTTC |
