Fig. 3: STC1 promotes angiogenesis in breast cancer lung metastases.
From: Stanniocalcin 1 promotes lung metastasis of breast cancer by enhancing EGFR–ERK–S100A4 signaling
A, B IHC analyses of α-SMA+, CD31+, and LY6G+ cells in the lung metastases of breast cancer; n = 6 samples from three mice per group. C Tube formation of rhSTC1-treated HUVECs. D Tube formation of STC1-overexpressing HUVECs. E Tube formation of HUVECs co-cultured with rhSTC1-pretreated MDA-MB-231. F Tube formation of HUVECs co-cultured with STC1-overexpressing MDA-MB-231 or STC1-downregulated LM2. The tube-forming capacity of HUVECs was evaluated by vessel length, percentage of vessel area, and the number of vascular junction points in (C–F). G Relative mRNA expression levels of VEGFA in HUVECs under corresponding treatment or co-culture conditions. The p values were obtained by unpaired t-test. Scale bars, 100 μm. STC1 stanniocalcin 1, IHC immunohistochemistry, rhSTC1 recombinant human STC1. ns no significance, p > 0.05; **p < 0.01; ***p < 0.001.
