Fig. 2: Effect of Nrh-L/R variants on cell death induced by Ca2+ stress inducers (MCF-7 breast cancer cells). | Cell Death & Disease

Fig. 2: Effect of Nrh-L/R variants on cell death induced by Ca2+ stress inducers (MCF-7 breast cancer cells).

From: Nrh L11R single nucleotide polymorphism, a new prediction biomarker in breast cancer, impacts endoplasmic reticulum-dependent Ca2+ traffic and response to neoadjuvant chemotherapy

Fig. 2

A Western blotting. Nrh expression in MCF-7 cells transfected with the pCSII empty vector or pCSII vector harboring Flag-tagged Nrh-R or Nrh-L ORFs (50 µg of cell lysate). Vinculin was used for calibration. B Cell death quantification (SytoxGreenTM-positive cells), in MCF-7 cells transfected with pCSII empty vector, pCSII Flag Nrh-L or pCSII Flag Nrh-R. Histograms show cell death expressed as mean ± SEM (three independent experiments) in the following conditions: Thapsigargin (Thapsi) 10 μM for 80 h (top panel) or Tunicamycin (Tunica) 2 μM for 80 h (bottom panel).

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