Fig. 2: LINC01594 inhibits CELF6 expression. | Cell Death & Disease

Fig. 2: LINC01594 inhibits CELF6 expression.

From: Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis

Fig. 2

A, B The volcano map (A) and MA map (B) showed 1102 genes positively and 611 genes negatively associated with LINC01594 treatment. C GO classification map showing biological process cellular components and molecular functions associated with LINC01594. D KEGG classification map showing the most related genetic information processing with LINC01594. E Top 10 differentially expressed mRNAs positively and negatively correlated with LINC01594. F The top ten genes with positive and negative correlation differences with LINC01594 were taken from the GO annotated genes associated with binding. Among them, we identified the genes related to splicing function. G qRT‒PCR detected the mRNA level in SW480 and LoVo cells. (n = 3). *P < 0.05, **P < 0.01. H, I Western blotting was used to determine the levels of CELF6 in LINC01594-overexpressing and LINC01594-silenced SW480 cells (H) and LoVo cells (I). J, K IF assay showed that overexpression of LINC01594 inhibited CELF6 expression and knockdown of LINC01594 promoted CELF6 expression in SW480 cells (J) and LoVo cells (K). IF intensity statistical data are shown. (n = 3). *P < 0.05, **P < 0.01.

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