Fig. 3: Efferocytosis by MSCs decreased expression of metabolism genes and increases stress response genes. | Cell Death & Disease

Fig. 3: Efferocytosis by MSCs decreased expression of metabolism genes and increases stress response genes.

From: Efferocytosis by bone marrow mesenchymal stromal cells disrupts osteoblastic differentiation via mitochondrial remodeling

Fig. 3

GSEA analysis of efferocytic ST2 cell (PMN+) vs control at 3 h (A, C, E) and at 24 h (B, D, F). Gene signatures for the (A, B) processing machinery, (C, D) metabolic pathways and biogenesis, and (E, F) cellular fate. G ST2 cells incubated with excess (1:10) of end stage neutrophils (PMN) for 24 h and then stained for Beta-galactosidase (β-Gal) and analyzed via flow cytometry. Graphs show N = 9 wells (3 wells/experiment), quantified showing Mean + SD. **p < 0.01, ***p < 0.001, (ANOVA with post Tukey test). H ST2 cells challenged with excess neutrophils (1:10) for 24 h and then sorted alongside controls into PMN +/− and replated and counted daily over a 5-day timespan. Graphs show N = 4 wells, quantified showing Mean + SD. **p < 0.01, ****p < 0.0001, (ANOVA with post Tukey test).

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