Fig. 5: Efferocytosis of end stage neutrophils disrupts adipocytic differentiation. | Cell Death & Disease

Fig. 5: Efferocytosis of end stage neutrophils disrupts adipocytic differentiation.

From: Efferocytosis by bone marrow mesenchymal stromal cells disrupts osteoblastic differentiation via mitochondrial remodeling

Fig. 5

A Experimental Schematic (B, C) Bone marrow-derived ST2 cells incubated with increasing concentrations end stage neutrophils (PMN) for 24 h, then differentiated into adipocytes using supplemented media for 21 days and stained for lipid deposits with BODIPY staining. Representative images of 3, quantified showing Mean + SD. ***p < 0.001, (ANOVA). D Experimental Schematic for sorted samples (E, F) ST2 cells challenged with excess neutrophils (1:10) and then sorted alongside controls into PMN +/−, differentiated into adipocytes using supplemented media for 21 days, and then stained for lipid deposits with BODIPY staining. Representative Images of 3, quantified showing Mean+SD. **p < 0.01, ***p < 0.001, (ANOVA with post Tukey test). G Heat map of genes related to adipogenesis at 3 h or 24 h vs the control.

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