Fig. 2: LINC00839 facilitates GSC maintenance and radiation resistance in vitro and in vivo. | Cell Death & Disease

Fig. 2: LINC00839 facilitates GSC maintenance and radiation resistance in vitro and in vivo.

From: METTL3-mediated m6A modification of LINC00839 maintains glioma stem cells and radiation resistance by activating Wnt/β-catenin signaling

Fig. 2

A GSCs were transfected with Vector, LINC00839, control ASO, or LINC00839 ASO separately. Effects on self-renewal were assessed by in vitro extreme limiting dilution assays (ELDA) for sphere formation.* refers to compare between the Vector group and the LINC00839 group; # refers to compare between the control ASO group and LINC00839 ASO group. **p < 0.001, ##p < 0.001. B GSCs were transfected with Vector, LINC00839, control ASO, or LINC00839 ASO separately. Representative images of spheres were photographed. C GSCs were transfected with Vector, LINC00839, control ASO, or LINC00839 ASO separately. Cells were treated with or without IR (0–10 Gy) and seeded in 10 cm dishes. Colonies were counted after 2 weeks, and the surviving fraction was calculated as the ratio of the plating efficiency of the treated cells to that of control cells. **p < 0.001. D GSCs were transfected with control ASO or LINC00839 ASO. The apoptotic rates were measured by flow cytometry. **p < 0.001. E Representative images of hematoxylin and eosin stained cross-sections of tumor-bearing brains harvested after transplantation of transfected GSCs with or without IR treatment. F Kaplan–Meier survival curves of immunocompromised mice-bearing intracranial injected GSCs. **p < 0.001. G, H Representative IHC images and the quantification of Ki-67 (G) and γ-H2AX (H) were shown. Scale bars, 60 μm. **p < 0.001. I Representative TUNEL images and the quantification were shown. Scale bars, 60 μm. **p < 0.001.

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