Fig. 4: YTHDF2 stabilizes LINC00839. | Cell Death & Disease

Fig. 4: YTHDF2 stabilizes LINC00839.

From: METTL3-mediated m6A modification of LINC00839 maintains glioma stem cells and radiation resistance by activating Wnt/β-catenin signaling

Fig. 4

A FISH and IF double staining in GSCs shows the co-localization of LINC00839 (Cy3; Red) and YTHDF2 (Green); Nuclei are stained blue (DAPI). Scale bar: 10 μm. B LINC00839 expression in GSCs transfected with control or YTHDF2 shRNA was detected by qRT-PCR. **p < 0.001. C LINC00839 expression in GSCs transfected with vector or YTHDF2 was detected by qRT-PCR. **p < 0.001. D LINC00839 RNA stability in control and YTHDF2-silenced cells. qRT-PCR of LINC00839 at the indicated time points after treatment with actinomycin D (10 μg/mL). E YTHDF2 was immunoprecipitated and RIP-qPCR was used to assess the association of LINC00839 with YTHDF2. **p < 0.001. F Enrichment of LINC00839 in the immunoprecipitated RNA fraction of GSCs following overexpression of either wildtype (WT) YTHDF2 or m6A-binding mutant YTHDF2 (W432A and W486A) in GSCs. **p < 0.001. G LINC00839 expression in GSCs expressing either WT or mutant YTHDF2 was detected by qRT-PCR. **p < 0.001.

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