Fig. 3: Expression of BCKDK affects the talin1 expression levels in breast cancer cells. | Cell Death & Disease

Fig. 3: Expression of BCKDK affects the talin1 expression levels in breast cancer cells.

From: BCKDK regulates breast cancer cell adhesion and tumor metastasis by inhibiting TRIM21 ubiquitinate talin1

Fig. 3

A, B The expression of talin1 and talin2 were detected by western blot in cells of BCKDK overexpression or knockdown. C IF assay showed the colocalization of talin1 and BCKDK in MCF-7 cells. Cells were stained for BCKDK (red) and talin1 (green). Scale bar, 50 μm. D, E BCKDK was coimmunoprecipitated with talin1 in MCF-7 and MDA-MB-231 cells. F Exogenous Talin1 coimmunoprecipitated with BCKDK in HEK293T cells. G The ubiquitination of talin1 was examined by western blot in HEK293T cells.

Back to article page