Fig. 3: ARRB2 interacts with TRAF6 and BECN1. | Cell Death & Disease

Fig. 3: ARRB2 interacts with TRAF6 and BECN1.

From: β‐arrestin 2 negatively regulates lung cancer progression by inhibiting the TRAF6 signaling axis for NF-κB activation and autophagy induced by TLR3 and TLR4

Fig. 3

A HEK-293T cells were transfected with mock, Myc-TRAF6, and Flag-ARRB2. Immunoprecipitation (IP) assay was performed with an anti-Myc antibody. IP complexes were immune-probed with anti-Myc and anti-Flag antibodies. B Truncated mutants of TRAF6 were generated as described in Materials and Methods. C HEK-293T cells were transfected with mock, Myc-ARRB2, Flag-TRAF6 wild type (WT), and Flag-truncated mutants of TRAF6 as indicated. IP assay was performed with an anti-Myc antibody. IP complexes were immune-probed with anti-Myc and anti-Flag antibodies. D A schematic model of the interaction between TRAF6 and ARRB2. E HEK-293T cells were transfected with mock, Myc-BECN1, and Flag-ARRB2 and IP assay was performed with an anti-Myc antibody. IP complexes were immune-probed with anti-Myc and anti-Flag antibodies. F Truncated mutants of BECN1 were generated as described in Material and Methods. G HEK-293T cells were transfected with mock, Myc-ARRB2, Flag-BECN1 wild type (WT), and Flag-truncated mutants of BECN1 as indicated. IP assay was performed with an anti-Flag antibody. IP complexes were immune-probed with anti-Myc and anti-Flag antibodies. H A schematic model of the interaction between BECN1 and ARRB2. I A molecular model of the interaction between ARRB2 and TRAF6 or BECN1.

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