Fig. 6: ARRB2KO H1299 cells exhibit enhanced cell migration and invasion induced by TLR3 and TLR4. | Cell Death & Disease

Fig. 6: ARRB2KO H1299 cells exhibit enhanced cell migration and invasion induced by TLR3 and TLR4.

From: β‐arrestin 2 negatively regulates lung cancer progression by inhibiting the TRAF6 signaling axis for NF-κB activation and autophagy induced by TLR3 and TLR4

Fig. 6

A, B Ctrl H1299 and ARRB2KO H1299 cells were treated with vehicle (DMSO, 0.1% v/v concentration), Poly I:C (20 μg/ml), or LPS (15 μg/ml) in the presence or absence of 3-MA (5 mM) for different time periods (A), as described in Materials and Methods. The residual gap between migrating cells from the opposing wound edge is expressed as a percentage of the initial scraped area (±SD, n = 3 different plates) (B). *p < 0.05, **p < 0.01, and ***p < 0.001. C, D Ctrl H1299 and ARRB2KO H1299 cells were treated with vehicle (DMSO, 0.1% v/v concentration), Poly I:C (15 μg/ml), or LPS (20 μg/ml) in the presence or absence of 3-MA (5 mM) (C), as described in Material and Methods. The number of migrating cells were counted. Results are presented as the mean ± SD of three independent experiments (D). *p < 0.05, **p < 0.01, and ***p < 0.001.

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