Fig. 7: ARRB2KO H1299 cells exhibit enhancements of colony formation and proliferation by TLR3 and TLR4. | Cell Death & Disease

Fig. 7: ARRB2KO H1299 cells exhibit enhancements of colony formation and proliferation by TLR3 and TLR4.

From: β‐arrestin 2 negatively regulates lung cancer progression by inhibiting the TRAF6 signaling axis for NF-κB activation and autophagy induced by TLR3 and TLR4

Fig. 7

A, B Ctrl H1299 and ARRB2KO H1299 cells were treated with vehicle (DMSO, 0.1% v/v concentration), Poly I:C (25 μg/ml), or LPS (25 μg/ml) in the presence or absence of 3-MA (5 mM). Anchorage-dependent colony formation assay was performed (A), as described in Materials and Methods. The number of colonies was measured using Adobe Photoshop software, and results are presented as mean ± SD of three independent experiments (B, n = 3 plates). *p < 0.05, **p < 0.01, and ***p < 0.001. C, D Ctrl H1299 and ARRB2KO H1299 cells were treated with vehicle (DMSO, 0.1% v/v concentration), Poly I:C (20 μg/ml), or LPS (20 μg/ml) in the presence or absence of 3-MA (5 mM). Anchorage-independent colony formation assay was performed (C), as described in Materials and Methods. D The number of colonies was measured using Adobe Photoshop software, and results are presented as mean ± SD of three independent experiments (D, n = 3 plates). *p < 0.05, **p < 0.01, and ***p < 0.001. Ctrl H1299 and ARRB2KO H1299 cells (E, Poly I:C; F, LPS) or Ctrl A549 and ARRB2KO A549 cells (G, Poly I:C; H, LPS) were treated with vehicle (WO, DMSO, 0.1% v/v concentration), Poly I:C (1 μg/ml), or LPS (7 μg/ml). MTT assay was then performed, as described in in Material and Methods. Results are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001.

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