Fig. 3: MGCG facilitates GBM progression by promoting the expression of ATG2A in LN229 cells.
From: MGCG regulates glioblastoma tumorigenicity via hnRNPK/ATG2A and promotes autophagy
A RT‒qPCR analysis of the relative expression of MGCG and ATG2A mRNA in GBM tissue samples. B Western blot analysis was used to detect the protein levels of p62 and LC3 after ATG2A knockdown in LN229 cells. C RT‒qPCR analysis of the relative expression of ATG2A after MGCG knockdown in LN229 cells. D Western blot analysis of the protein levels of ATG2A after MGCG overexpression or knockdown in LN229 cells. E The expression of ATG2A was assayed in the normal and GBM tissues by RT‒qPCR (normal n = 27, GBM n = 42). F Kaplan‒Meier plots of overall survival of GBM patients with high and low levels of ATG2A. G The data of RT‒qPCR on the expression of ATG2A in LN229 cells treated with si-NC and si-ATG2A. H Western blot was used to detect the ATG2A protein level after treated with si-NC and si-ATG2A in LN229 cells. I The data of RT‒qPCR on the expression of ATG2A in U251 cells treated with si-NC and si-ATG2A. J Western blot was used to detect the ATG2A protein level after treated with si-NC and si-ATG2A in U251 cells. K Colony formation assays were used to detect the effect of ATG2A knockdown on the growth of LN229 cells. L The data of Transwell analysis show the effect of ATG2A knockdown on the migration of LN229 cells. M The data of TUNEL analysis show the effect of ATG2A knockdown on apoptosis of LN229 cells. N Western blot analysis of the protein levels of p62 and LC3 after MGCG overexpression or overexpression and simultaneous knockdown of ATG2A. O Colony formation assays were used to detect the effect of MGCG overexpression or overexpression and simultaneous knockdown of ATG2A on the growth of LN229 cells. P The data of Transwell analysis show the effect of MGCG overexpression or overexpression of MGCG and simultaneous knockdown of ATG2A on the migration of LN229 cells. Q The data of TUNEL analysis show the effect of MGCG overexpression or overexpression and simultaneous knockdown of ATG2A on apoptosis of LN229 cells. Error bars, SEM from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 by two-tailed Student’s test.
