Fig. 2: In vivo delivery of miR-200c-3p inhibits fibrosis and preserves the number of ATI cells in the lung.

A Representative images of whole lung sections stained with Masson-Trichrome to visualize collagen fibers in blue at 30 days after the administration of the indicated treatments. B Quantification of pulmonary fibrosis using the Ashcroft score. C Quantification of the area covered by α-SMA+ activated fibroblasts. D Quantification of the percentage of Podoplanin+ cells. E Representative immunofluorescence images of lung sections stained for α-SMA. Nuclei were counterstained with Hoechst (blue). F Representative immunofluorescence images of lung sections stained for Pro-Spc (white) and Podoplanin (Pdln, green) to label ATII and ATI cells, respectively. Nuclei were counterstained with Hoechst (blue). Scale bar in A, 1 mm; in E, F, 100 μm. Data in B–D are shown as mean ± S.E.M (n ≥ 3). Statistical significance was determined using one-way ANOVA, *P < 0.05, ****P < 0.0001.