Fig. 4: Endothelial Flt1 depletion in endothelial cells improves ATII transdifferentiation.

A Representative immunofluorescence images of control ATII cells conditioned with wt or Flt1 KO ECs supernatants, stained for Rage (red). Nuclei were counterstained with Hoechst (blue). B Quantification of fully differentiated (>1500 µm²) Rage⁺ ATI cells, expressed as a percentage of Rage⁺ cells, in the indicated conditions; n = 11. C. Quantification of fully differentiated (>1500 µm²) Rage⁺ ATI cells from bleomycin-treated mice, expressed as a percentage of Rage⁺ cells, in the indicated conditions; n = 10. D Representative immunofluorescence images of ATII cells from bleomycin-treated mice, conditioned with wt or Flt1 KO ECs supernatants, stained for Rage (red). Nuclei were counterstained with Hoechst (blue). E Quantification of fully differentiated (>1500 µm²) Rage⁺ ATI cells, expressed as a percentage of Rage⁺ cells, in the indicated conditions; n = 10. F Quantification of fully differentiated (>1500 µm²) Rage⁺ ATI cells from bleomycin-treated mice, expressed as a percentage of Rage⁺ cells, in the indicated conditions; n = 10. G Representative immunofluorescence images of control ATII cells in contact with wt or Flt1 KO ECs cells, stained for Rage (red) and Erg (green), respectively. Nuclei were counterstained with Hoechst (blue). H Representative immunofluorescence images of ATII cells from bleomycin-treated mice in contact with wt or Flt1 KO ECs cells, stained for Rage (red) and Erg (green), respectively. Nuclei were counterstained with Hoechst (blue). Scale bar in A, D, G, H, 100 μm. Data in B, C, E, and F are shown as mean ± S.E.M (n = 10). Statistical significance was determined using one-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.