Fig. 3: Apoptotic exosome-like vesicles are internalized by a nonclassical endocytosis pathway. | Cell Death & Disease

Fig. 3: Apoptotic exosome-like vesicles are internalized by a nonclassical endocytosis pathway.

From: Apoptotic exosome-like vesicles transfer specific and functional mRNAs to endothelial cells by phosphatidylserine-dependent macropinocytosis

Fig. 3

A Quantification of ApoExo, ApoBodies, and ExoN uptake and transferrin (Tfn) uptake in serum-starved endothelial cells pretreated for 30 min with monodansylcadaverine 400 µM or its vehicle (DMSO; Ctrl) followed by a treatment of 1 h, as determined by flow cytometry. n = 3 for each condition. B Serum-starved endothelial cells were transfected with Ctrl or 90 nM CAV1 siRNA and exposed to labeled ApoExos, ApoBodies, or ExoN for 1 h. Quantification of EV uptake by flow cytometry. Representative immunoblot after CAV1 knockdown is depicted in Fig. S3. n = 3 for each condition. C Serum-starved endothelial cells were transfected with Ctrl or 90 nM CAV1 siRNA and then pretreated for 30 min with 400 µM monodansylcadaverine or a vehicle (DMSO; Ctrl) followed by a 1 h treatment with labeled ApoExos, ApoBodies or ExoNs. Quantification of EV uptake by flow cytometry. n = 3 for each condition. Flow cytometry experiments expressed as the cell fluorescence percentage of vehicle-treated cells MFI (30,000 events/sample) ± SEM. P values were obtained by unpaired t test (*P < 0.05, **P < 0.01, and ***P < 0.001).

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