Fig. 3: PCa exosome PGAM1 promotes angiogenesis in HUVECs in vitro.
From: Exosomal PGAM1 promotes prostate cancer angiogenesis and metastasis by interacting with ACTG1
HUVECs were treated with exosomes isolated from the DU145-CM for 24 h prior to the following assays. Controls were treated with an equal volume of PBS. A, B An in vitro Matrigel tube formation assay and CAM assays was performed to assess the angiogenic capacity of HUVECs. And The number of branches per highpower field was analyzed. The scale bar represents 200 μm. C Colony-formation assays were performed to assess the proliferative capacity of HUVECs. D Transwell assay was performed to evaluate the invasive ability of HUVECs. The scale bar represents 200 μm. E Wound healing assays were performed to assess the migration ability of HUVECs. The scale bar represents 100 μm. (Error bars represent means ± SD; ***P < 0.001).
