Fig. 4: PGAM1 and ACTG1 bind in HUVECs.
From: Exosomal PGAM1 promotes prostate cancer angiogenesis and metastasis by interacting with ACTG1
A GST-pulldown enriched protein silver staining assay. B Venn diagram of GST and GST-PGAM1 differential proteins. C GSEA pathway enrichment map of GST-PGAM1 pull-down proteins. D Scoring ranking of GST-PGAM1 pull-down proteins. E The expression correlation between PGAM1 and ACTG1 was inferred by bioinformatics. (GEPIA 2 (cancer-pku.cn)). F, G Co-IP and western blotting assays demonstrated the binding of PGAM1 and ACTG1 in HUVECs and DU145 cells without any intervention. H Western blotting analysis of PGAM1 and ACTG1 in HUVECs after co-culture with exosomes secreted by DU145-CM Controls were treated with an equal volume of PBS. I IF was used to analyze the co-localization of PGAM1 (green), ACTG1 (red) and DAPI (blue) in HUVECs and DU145 cells. Co-localization indicates the co-localization analysis of PGAM1 and ACTG1 within HUVECs and PCa cells. The scale bar represents 15 μm.
