Fig. 5: The PCa exosome PGAM1 promotes the production of podosomes in HUVECs.
From: Exosomal PGAM1 promotes prostate cancer angiogenesis and metastasis by interacting with ACTG1
HUVECs were treated with exosomes isolated from the DU145-CM before to the following assays. Controls were treated with an equal volume of PBS. A Co-localization of F-actin (red), Cortactin (green) and DAPI (blue) was detected by IF in HUVECs to indicate podosomes. The scale bar represents 10 μm. B Quantification of podosomes in HUVECs. C HUVECs were plated on FITC-gelatin (green) and stained for F-actin (red) and DAPI (blue). The area of gelatin degradation is shown as a black area below the cells. The scale bar represents 50 μm. D Quantification of FITC-gelatin degradation in DU145 cells. E F-actin (red), Cortactin (green) and DAPI (blue) staining was used to perform in vitro Matrigel tube formation assays. The scale bar represents 150 μm. F Quantification of podosomes in HUVECs. G 3D z-stack acquisition was performed in the gelatin degradation experiment, and orthogonal views of the x-z plane and y-z plane are given. The red arrow indicates the area of gelatin degradation. The scale bar represents 5 μm. (Error bars represent means ± SD; **P < 0.01, ***P < 0.001).
