Fig. 6: Reduced invadopodia formation in PCa cells with knockdown of PGAM1.
From: Exosomal PGAM1 promotes prostate cancer angiogenesis and metastasis by interacting with ACTG1
A Co-localization of F-actin (red), Cortactin (green) and DAPI (blue) was detected by IF in DU145 cells to indicate invadopodia. The scale bar represents 10 μm. B Quantification of invadopodia in DU145 cells. C Quantification of FITC-gelatin degradation in DU145 cells. D DU145-NC-PGAM1 or DU145-SH1-PGAM1 cells were plated on FITC-gelatin (green) and stained for F-actin (red) and DAPI (blue). The scale bar represents 50 μm. E PC3-NC-PGAM1 or PC3-SH1-PGAM1 cells were plated on FITC-gelatin (green) and stained for F-actin (red), Cortactin and DAPI (blue). The scale bar represents 10 μm. F Co-localization of F-actin (red), Cortactin (green) and DAPI (blue) was detected by IF in PC3 cells to indicate Invadopodia. The scale bar represents 10 μm. G Quantification of invadopodia in PC3 cells. H Quantification of FITC-gelatin degradation in PC3 cells. (Error bars represent means ± SD; ***P < 0.001).
