Fig. 1: NUCKS1 promoted osteosarcoma cell growth and migration in vitro and in vivo.

A NUCKS1 was knocked down in 143B and MNNG/HOS cells. The expression levels of NUCKS1 were detected by western blot. B, C 143B and MNNG/HOS cells with or without NUCKS1 depletion were tested for cell growth in the colony formation assay. After 1 week, viable colonies were counted and are shown (B). Data are depicted as bar graphs (C). D, E The migration of the indicated cells was detected by Transwell assays. Representative images of crystal violet-stained culture plates are shown (D). Data are depicted as bar graphs (E). F NUCKS1 was overexpressed in 143B and MNNG/HOS cells. The expression levels of NUCKS1 were detected by Western blot. G, H 143B and MNNG/HOS cells with or without NUCKS1 overexpression were tested for cell growth in the colony formation assay. After 1 week, viable colonies were counted and are shown (G). Data are depicted as bar graphs (H). I, J The migration of the indicated cells was detected by Transwell assays. Representative images of crystal violet-stained culture plates are shown (I). Data are depicted as bar graphs (J). K–M 143B cells (10⁶ cells per mouse) with or without NUCKS1 knockdown were injected subcutaneously into nude mice (n = 6). Representative images of xenograft tumors (K). The weight (L) and volume (M) of the tumors were calculated and analyzed. N Tumor tissue sections were analyzed by IHC using anti-Ki-67 and anti-Cleaved Caspase-3 antibodies. O, P 143B cells (10⁶ cells per mouse) with or without NUCKS1 knockdown were injected intravenously into nude mice (n = 5 per group). HE staining are displayed (O). Each group of metastatic nodules was assessed (P). Data in C, E, H, J, L, M and P were analyzed by Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001.