Fig. 3: Knockdown of LEF1 or KDM4A suppresses proliferation but promotes apoptosis in CAL-27 and SCC-9 cells in vitro. | Cell Death & Disease

Fig. 3: Knockdown of LEF1 or KDM4A suppresses proliferation but promotes apoptosis in CAL-27 and SCC-9 cells in vitro.

From: Carcinogenesis promotion in oral squamous cell carcinoma: KDM4A complex-mediated gene transcriptional suppression by LEF1

Fig. 3

A Twenty-four hour after transfection of LEF1 or KDM4A siRNA, the CAL-27 or SCC-9 cells were incubated with EdU for 3 h. A fluorescence microscope was used to detect EdU. Scale bar: 100 μm. B Cell viability of CAL-27 and SCC-9 48 h after transfection, as assessed by CCK-8. C Expression of apoptosis markers measured using RT-PCR in LEF1/KDM4A-depleted CAL-27 and SCC-9 cells. Results are expressed as fold-change relative to control, and GAPDH was used as negative control. D Expression of apoptosis markers was measured using western blotting (WB) in LEF1/KDM4A-depleted CAL-27 and SCC-9 cells. GAPDH served as a loading control. E Apoptosis rate of LEF1/KDM4A-depleted CAL-27 cells assessed by flow cytometry. F Cell cycle analysis of LEF1/KDM4A-depleted CAL-27 cells assessed by flow cytometry. Data are shown as means ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

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