Fig. 4: scRNA-seq profiling of immune cell components in melanoma.

A. t-SNE plot showing the clusters of immune cells and cell origins by color, according to immune cell types. B The cell classification basis of immune cell scRNA-seq analysis was displayed with the marker genes expression in Feature plot. C Pseudotime-ordered analysis to construct the developmental trajectories of the isolated immune cell subgroups. D WGCNA in bulk sequence. Gene cluster dendrogram clustered by weighted gene co-expression network in different colors. E The heatmap of module-trait relationships. The number in and outside the bracket represents the P-value and Pearson coefficient, respectively. F Representative IHC stanning of CD8⁺ and CD57⁺ in immune cell from the clinic. Scale bar = 100 μm, n = 4 samples per group. G The quantification of PRGs protein in epidermal areas, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, compared with the control group.