Fig. 2: RC48 inhibited the growth of HER2-positive colorectal cancer cells in vitro.

A In vitro growth inhibition assay for HCT116 cells treated with MMAE and Paclitaxel for 24 h. B In vitro growth inhibition assay for HCT116 and HT29 cells. C In vitro growth inhibition assay for MC38HER2 and MC38Ctrl cells treated with RC48 for 48 h. D In vitro growth inhibition assay for MC38Ctrl and MC38HER2 cells treated with MMAE for 24 h. E Flow cytometry analysis of cell cycle after treatment with different concentrations of RC48 for 48 h. F Clonogenic assay of HCT116, CT26HER2 and MC38HER2 treated with RC48. Data represent the mean ± SEM of three replicates. *P < 0.05, **P < 0.01, versus as indicated. n.s. not significant.