Fig. 3: RC48 triggered cGAS-STING activation in colon cancer cells. | Cell Death & Disease

Fig. 3: RC48 triggered cGAS-STING activation in colon cancer cells.

From: A HER2-targeting antibody-MMAE conjugate RC48 sensitizes immunotherapy in HER2-positive colon cancer by triggering the cGAS-STING pathway

Fig. 3

A–C HCT116 cells were treated with different concentrations of RC48, MMAE, or Disitamab (0, 10, 30, 100 μg/ml) for 48 h, and the expression of p-TBK1, p-IRF3, TBK1, IRF3 protein was detected by immunoblotting. D The expression of the IRF3-responsive genes, CXCL10, IFN-β, and IFIT1 in HCT116 treated with 100 μg/ml RC48 for 48 h and 30 μM CPT11 for 12 h are detected by qPCR. *P < 0.05, **P < 0.01, versus as indicated. n.s. not significant.

Back to article page