Fig. 3: OGDHL inhibits the progress of ccRCC in vitro. | Cell Death & Disease

Fig. 3: OGDHL inhibits the progress of ccRCC in vitro.

From: The m6A modification-mediated OGDHL exerts a tumor suppressor role in ccRCC by downregulating FASN to inhibit lipid synthesis and ERK signaling

Fig. 3

The results are expressed as the mean ± SEM of three independent experiments, and there are at least three replicates in each independent experiment. t-test, ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05 (independent-samples t-test for statistics). A Western blotting and qPCR were used to verify the overexpression of OGDHL at the protein and mRNA levels, respectively. B Western blotting and qPCR confirmed the knockdown of OGDHL at the protein and mRNA levels, respectively. C Colony formation experiments of ccRCC cell lines infected with OGDHL overexpressing and control lentivirus. D The CCK8 assay was used to determine the level of proliferation of OGDHL overexpressing and control ccRCC cell lines. E The CCK8 assay was used to determine the level of proliferation of knockdown OGDHL and control ccRCC cell lines. F Transwell assays were used to determine the migration and invasion abilities of OGDHL-overexpressing and control ccRCC cell lines. G Transwell assays were used to determine the migration and invasion abilities of knockdown OGDHL and control ccRCC cell lines. H Flow cytometric analysis was performed on OGDHL overexpressing and control ccRCC cell lines. Representative images and quantification of results are presented. I Tunel fluorescence staining was used to reflect the apoptosis level of OGDHL overexpression and control ccRCC cell lines. J Tunel fluorescence staining was used to reflect the apoptosis level of OGDHL knockdown and control ccRCC cell lines.

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