Fig. 7: The impact of Plk1 on ATP6V phosphorylation and the role of autophagy on fibroblast activation. | Cell Death & Disease

Fig. 7: The impact of Plk1 on ATP6V phosphorylation and the role of autophagy on fibroblast activation.

From: Plk1 promotes renal tubulointerstitial fibrosis by targeting autophagy/lysosome axis

Fig. 7

A, B Western blot analysis of ATP6V1A in NRK49F cells treated with BI6727 in the presence or absence of TGF-β1. n = 3. C Immunoprecipitated ATP6V1A was analyzed by immunoblotting (IB) with antibodies against p-Ser and p-Thr after treatment with different concentrations of BI6727. n = 3. D, E Western blot analysis of FN in NRK49F cells treated with or without Baf-A1/CQ in the presence of TGF-β1. n = 3. F, G Western blot analysis of FN in NRK49F cells treated with Concanamycin A or ATP6V1A-shRNA in the presence of TGF-β1. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001.

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