Fig. 5: MCC950 reverses the inhibitory effect of Sorcin knockdown on the proliferation, migration, and invasion ability of HCC cells. | Cell Death & Disease

Fig. 5: MCC950 reverses the inhibitory effect of Sorcin knockdown on the proliferation, migration, and invasion ability of HCC cells.

From: Sorcin regulate pyroptosis by interacting with NLRP3 inflammasomes to facilitate the progression of hepatocellular carcinoma

Fig. 5

A, B HCC-LM3 and HuH7 cells of Sorcin knockdown with shRNA interference were pre-treated with 10 μmol MCC950 for 24 h. Western blot results demonstrated that by blocking NLRP3 with MCC950, Sorcin inhibition-induced upregulation of Caspase-1, GSDMD, IL-1β, and IL-18 proteins was notably inhibited in HCC-LM3 and HuH7 cells. C, D The CCK8 assays showed that knockdown Sorcin suppressed the proliferation of HCC-LM3/HuH7 cells, while MCC950 partially restored the inhibitory effect of Sorcin knockdown on the proliferation capacity of HCC cells. E Transwell assays revealed that NLRP3 inhibition by MCC950 promoted cell migration in HCC cell lines. F Cell wound-healing proved that suppression of NLRP3 by MCC950 enhanced the ability of cell migration in HCC-LM3 and HuH7 cells at 0 h, 24 h, and 48 h. J The transwell invasion assays manifested that NLRP3 inhibitor MCC950 can reverse the inhibitory effect of Sorcin knockdown on the invasion ability of HCC cells. All: *P < 0.05, **P < 0.01, ****P < 0.0001, and ns no significance.

Back to article page