Fig. 1: Ctsk-Cre+;Dlk2fl/fl mice acquire a high-bone-mass phenotype.
From: Dlk2 interacts with Syap1 to activate Akt signaling pathway during osteoclast formation
A Representative 3D micro-CT reconstruction images of distal femurs from 8-week-old Ctsk-Cre-;Dlk2fl/fl (WT) and Ctsk-Cre+;Dlk2fl/fl male mice. B Quantitative micro-CT analysis of the images shown in (A); BT/TV, bone volume percentage/tissue volume ratio; Tb.N, trabecular bone number; Tb.Sp, trabecular bone separation; Tb.Th, trabecular bone thickness; BMD, bone mineral density; SMI, structure model index. C Von Kossa staining of undecalcified tibial sections from 8-week-old WT and Ctsk-Cre+;Dlk2fl/fl male mice. D Bone histomorphometric analysis of the Von Kossa-stained sections shown in C; BT/TV, bone volume percentage/tissue volume ratio; Tb.N, trabecular bone number; Tb.Dm, trabecular bone diameter. E TRAP staining of undecalcified tibial sections from 8-week-old WT and Ctsk-Cre+;Dlk2fl/fl male mice. F Bone histomorphometric analysis of the TRAP-stained sections shown in E; N.Oc/BS, osteoclast number/bone surface ratio; Oc.S/BS, osteoclast surface/bone surface ratio. G Double label staining with calcein and alizarin red of undecalcified tibial sections from 8-week-old WT and Ctsk-Cre+;Dlk2fl/fl male mice. H Bone histomorphometric analysis of the double label stained sections shown in G; MAR, mineral apposition rate; BFR/BS, bone formation rate. I Serum levels of CTX-I. J Serum levels of P1NP. (Scale bar, 100 μm, mean ± SD, Student’s tests, *p < 0.05, **p < 0.01, ***p < 0.001, n = 6 for each genotype).
